Data quality metrics extracted from 4wt8.cif.gz by aB_cif2table1 from BUSTER (Global Phasing Ltd.). See also the PDBx/mmCIF dictionary and a MXLIMS-compatible JSON representation.


Experimental information for 4WT8 at RCSB, PDBe, PDBj

Experiment
Method
_exptl.method
X-RAY DIFFRACTION
Experimental protocol
_diffrn_radiation.pdbx_diffrn_protocol
SINGLE WAVELENGTH
Source type
_diffrn_source.source
SYNCHROTRON
Source details
_diffrn_source.type
DIAMOND BEAMLINE I04
Synchrotron site
_diffrn_source.pdbx_synchrotron_site
Diamond
Beamline
_diffrn_source.pdbx_synchrotron_beamline
I04
Temperature [K]
_diffrn.ambient_temp
90
Detector technology
_diffrn_detector.detector
PIXEL
Collection date
_diffrn_detector.pdbx_collection_date
2013-06-14
Detector
_diffrn_detector.type
DECTRIS PILATUS 2M
Wavelength(s) [Å]
_diffrn_source.pdbx_wavelength_list
0.9795
Software
Data scaling
_software.classification
SCALA (3.3.21; 2013/01/04; Phil R. Evans; pre@mrc-lmb.cam.ac.uk)
Refinement
_software.classification
REFMAC (5.8.0073; Garib N. Murshudov; garib@ysbl.york.ac.uk)
General information
Spacegroup name
_symmetry.space_group_name_H-M
P 21 21 21
Unit cell parameters
_cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma}
214.21 457.45 639.66 90.0 90.0 90.0
Wavelength
_diffrn_radiation_wavelength.wavelength
0.97950 Å

Data quality metricsOverallInnerShellOuterShell
Low resolution limit [Å]
_reflns.d_resolution_low _reflns_shell.d_res_low
49.908 49.908 3.580
High resolution limit [Å]
_reflns.d_resolution_high _reflns_shell.d_res_high
3.400 10.750 3.400
Rmerge
_reflns.pdbx_Rsym_value _reflns_shell.Rmerge_I_obs
0.275 0.081 1.302
Rmeas
_reflns.pdbx_Rrim_I_all
0.298 - -
Rpim
_reflns.pdbx_Rpim_I_all _reflns_shell.pdbx_Rpim_I_all
0.112 0.028 0.608
Total number of observations
_reflns.pdbx_number_measured_all _reflns_shell.number_measured_all
5690971 238813 610257
Total number unique
_reflns.number_obs _reflns_shell.number_unique_all
841002 26853 121152
<I/σ(I)>
_reflns.pdbx_netI_over_sigmaI _reflns_shell.meanI_over_sigI_obs
8.60 3.80 0.50
Completeness [%]
_reflns.percent_possible_obs _reflns_shell.percent_possible_all
98.8 96.3 97.9
Multiplicity
_reflns.pdbx_redundancy _reflns_shell.pdbx_redundancy
6.8 8.9 5.0
  CC(1/2) - - -

Refinement
PDB entry ID
_entry.id
4WT8
Deposition date
_pdbx_database_status.recvd_initial_deposition_date
2014-10-29
Resolution
_refine.ls_d_res_low _refine.ls_d_res_high
49.9 - 3.400 Å
Rwork/Rfree
_refine.ls_R_factor_R_work _refine.ls_R_factor_R_free
0.2044 / 0.2412