| Experiment | |
|---|---|
Method _exptl.method | X-RAY DIFFRACTION |
Experimental protocol _diffrn_radiation.pdbx_diffrn_protocol | MAD |
Source type _diffrn_source.source | SYNCHROTRON |
Source details _diffrn_source.type | ALS BEAMLINE 8.2.1 |
Synchrotron site _diffrn_source.pdbx_synchrotron_site | ALS |
Beamline _diffrn_source.pdbx_synchrotron_beamline | 8.2.1 |
Temperature [K] _diffrn.ambient_temp | 100 |
Detector technology _diffrn_detector.detector | CCD |
Collection date _diffrn_detector.pdbx_collection_date | 2004-03-10 |
Detector _diffrn_detector.type | ADSC |
Wavelength(s) [Å] _diffrn_source.pdbx_wavelength_list | 0.9795,0.9796 |
| Software | |
Data reduction _software.classification | MOSFLM |
Data scaling #1 _software.classification | SCALA (CCP4 4.2) |
Data scaling #2 _software.classification | CCP4 (SCALA) |
Phasing #1 _software.classification | SOLVE |
Phasing #2 _software.classification | RESOLVE |
Model building _software.classification | RESOLVE |
Refinement _software.classification | REFMAC (5.2.0003) |
| General information | |
Spacegroup name _symmetry.space_group_name_H-M | C 2 2 21 |
Unit cell parameters _cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma} | 106.80 130.67 151.05 90.0 90.0 90.0 |
Wavelength _diffrn_radiation_wavelength.wavelength | 1.000000.979500.97960 Å |
| Data quality metrics | Overall |
|---|---|
Low resolution limit [Å] _reflns.d_resolution_low | 19.990 |
High resolution limit [Å] _reflns.d_resolution_high | 2.050 |
Rmerge _reflns.pdbx_Rmerge_I_obs | 0.076 |
| Rmeas | - |
| Rpim | - |
| Total number of observations | - |
Total number unique _reflns.number_obs | 66292 |
<I/σ(I)> _reflns.pdbx_netI_over_sigmaI | 12.50 |
Completeness [%] _reflns.percent_possible_obs | 99.9 |
Multiplicity _reflns.pdbx_redundancy | 4.9 |
| CC(1/2) | - |
| Refinement | |
|---|---|
PDB entry ID _entry.id | 1VL0 |
Deposition date _pdbx_database_status.recvd_initial_deposition_date | 2004-06-30 |
Resolution _refine.ls_d_res_low _refine.ls_d_res_high | 20.0 - 2.050 Å |
Rwork/Rfree _refine.ls_R_factor_R_work _refine.ls_R_factor_R_free | 0.1692 / 0.2070 |
Structure solution method _refine.pdbx_method_to_determine_struct | MAD |