Data quality metrics extracted from 7sks.cif.gz by aB_cif2table1 from BUSTER (Global Phasing Ltd.). See also the PDBx/mmCIF dictionary and a MXLIMS-compatible JSON representation.


Experimental information for 7SKS at RCSB, PDBe, PDBj

Experiment
Method
_exptl.method
X-RAY DIFFRACTION
Experimental protocol
_diffrn_radiation.pdbx_diffrn_protocol
SINGLE WAVELENGTH
Source type
_diffrn_source.source
SYNCHROTRON
Source details
_diffrn_source.type
APS BEAMLINE 31-ID
Synchrotron site
_diffrn_source.pdbx_synchrotron_site
APS
Beamline
_diffrn_source.pdbx_synchrotron_beamline
31-ID
Temperature [K]
_diffrn.ambient_temp
100
Detector technology
_diffrn_detector.detector
PIXEL
Collection date
_diffrn_detector.pdbx_collection_date
2019-02-27
Detector
_diffrn_detector.type
DECTRIS PILATUS3 6M
Wavelength(s) [Å]
_diffrn_source.pdbx_wavelength_list
0.97931
Software
Data processing #1
_software.classification
autoPROC (1.0.5 20210420)
Data processing #2
_software.classification
TRUNCATE (7.1.015)
Data reduction
_software.classification
XDS (Feb 5, 2021)
Data scaling
_software.classification
Aimless (0.7.7)
Phasing
_software.classification
PHASER
Refinement
_software.classification
BUSTER (2.10.4)
General information
Spacegroup name
_symmetry.space_group_name_H-M
P 65 2 2
Unit cell parameters
_cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma}
85.954 85.954 402.506 90.0 90.0 120.0
Wavelength
_diffrn_radiation_wavelength.wavelength
0.97931 Å

Data quality metricsOverallInnerShellOuterShell
Low resolution limit [Å]
_reflns.d_resolution_low _reflns_shell.d_res_low
19.998 19.998 2.585
High resolution limit [Å]
_reflns.d_resolution_high _reflns_shell.d_res_high
2.541 6.811 2.541
Rmerge
_reflns.pdbx_Rmerge_I_obs _reflns_shell.Rmerge_I_obs
0.143 0.056 6.616
Rmeas
_reflns.pdbx_Rrim_I_all _reflns_shell.pdbx_Rrim_I_all
0.144 0.056 6.706
Rpim
_reflns.pdbx_Rpim_I_all _reflns_shell.pdbx_Rpim_I_all
0.015 0.006 1.079
Total number of observations
_reflns.pdbx_number_measured_all _reflns_shell.number_measured_all
2554941 138489 55551
Total number unique
_reflns.number_obs _reflns_shell.number_unique_all
30225 1722 1461
<I/σ(I)>
_reflns.pdbx_netI_over_sigmaI _reflns_shell.meanI_over_sigI_obs
29.31 85.57 0.69
Completeness [%]
_reflns.percent_possible_obs _reflns_shell.percent_possible_all
100.0 100.0 100.0
Multiplicity
_reflns.pdbx_redundancy _reflns_shell.pdbx_redundancy
84.5 80.4 38.0
CC(1/2)
_reflns.pdbx_CC_half _reflns_shell.pdbx_CC_half
1.000 1.000 0.442
Anomalous completeness
_reflns.pdbx_percent_possible_anomalous _reflns_shell.pdbx_percent_possible_anomalous
100.0 100.0 100.0
Anomalous multiplicity
_reflns.pdbx_redundancy_anomalous _reflns_shell.pdbx_redundancy_anomalous
46.6 51.2 20.2
CC(ano)
_reflns.pdbx_CC_half_anomalous _reflns_shell.pdbx_CC_half_anomalous
-0.033 0.004 0.002
|DANO|/σ(DANO)
_reflns.pdbx_absDiff_over_sigma_anomalous _reflns_shell.pdbx_absDiff_over_sigma_anomalous
0.662 0.741 0.639

Refinement
PDB entry ID
_entry.id
7SKS
Deposition date
_pdbx_database_status.recvd_initial_deposition_date
2021-10-21
Resolution
_refine.ls_d_res_low _refine.ls_d_res_high
20.0 - 2.541 Å
Rwork/Rfree
_refine.ls_R_factor_R_work _refine.ls_R_factor_R_free
0.2306 / 0.2574
Structure solution method
_refine.pdbx_method_to_determine_struct
MOLECULAR REPLACEMENT
Starting model (for MR)
_refine.pdbx_starting_model
6BK6