Data quality metrics extracted from 5s9u.cif.gz by aB_cif2table1 from BUSTER (Global Phasing Ltd.). See also the PDBx/mmCIF dictionary and a MXLIMS-compatible JSON representation.


Experimental information for 5S9U at RCSB, PDBe, PDBj

Experiment
Method
_exptl.method
X-RAY DIFFRACTION
Experimental protocol
_diffrn_radiation.pdbx_diffrn_protocol
SINGLE WAVELENGTH
Source type
_diffrn_source.source
SYNCHROTRON
Source details
_diffrn_source.type
DIAMOND BEAMLINE I04-1
Synchrotron site
_diffrn_source.pdbx_synchrotron_site
Diamond
Beamline
_diffrn_source.pdbx_synchrotron_beamline
I04-1
Temperature [K]
_diffrn.ambient_temp
100
Detector technology
_diffrn_detector.detector
PIXEL
Collection date
_diffrn_detector.pdbx_collection_date
2021-01-12
Detector
_diffrn_detector.type
DECTRIS PILATUS 6M
Wavelength(s) [Å]
_diffrn_source.pdbx_wavelength_list
0.9126
Software
Data reduction
_software.classification
XDS
Data scaling
_software.classification
Aimless (0.7.4; 13/12/18; Phil Evans)
Phasing
_software.classification
REFMAC
Refinement
_software.classification
REFMAC (5.8.0267; Garib N. Murshudov; garib@ysbl.york.ac.uk)
General information
Spacegroup name
_symmetry.space_group_name_H-M
P 21 21 21
Unit cell parameters
_cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma}
79.96 108.51 111.68 90.0 90.0 90.0
Wavelength
_diffrn_radiation_wavelength.wavelength
0.91260 Å

Data quality metricsOverallInnerShellOuterShell
Low resolution limit [Å]
_reflns.d_resolution_low _reflns_shell.d_res_low
79.960 79.960 1.770
High resolution limit [Å]
_reflns.d_resolution_high _reflns_shell.d_res_high
1.730 7.740 1.730
Rmerge
_reflns.pdbx_Rmerge_I_obs _reflns_shell.Rmerge_I_obs
0.077 0.028 2.173
Rmeas
_reflns.pdbx_Rrim_I_all _reflns_shell.pdbx_Rrim_I_all
0.084 0.031 2.350
Rpim
_reflns.pdbx_Rpim_I_all _reflns_shell.pdbx_Rpim_I_all
0.032 0.012 0.888
Total number of observations
_reflns.pdbx_number_measured_all _reflns_shell.number_measured_all
679474 8043 51498
Total number unique
_reflns.number_obs _reflns_shell.number_unique_all
101875 1293 7461
<I/σ(I)>
_reflns.pdbx_netI_over_sigmaI _reflns_shell.pdbx_netI_over_sigmaI_obs
13.00 48.90 0.90
Completeness [%]
_reflns.percent_possible_obs _reflns_shell.percent_possible_all
100.0 99.7 100.0
Multiplicity
_reflns.pdbx_redundancy _reflns_shell.pdbx_redundancy
6.7 6.2 6.9
CC(1/2)
_reflns.pdbx_CC_half _reflns_shell.pdbx_CC_half
0.999 0.999 0.337

Refinement
PDB entry ID
_entry.id
5S9U
Deposition date
_pdbx_database_status.recvd_initial_deposition_date
2021-05-18
Resolution
_refine.ls_d_res_low _refine.ls_d_res_high
65.1 - 1.730 Å
Rwork/Rfree
_refine.ls_R_factor_R_work _refine.ls_R_factor_R_free
0.1915 / 0.2286
Structure solution method
_refine.pdbx_method_to_determine_struct
FOURIER SYNTHESIS
Starting model
_refine.pdbx_starting_model
6RB5