Data quality metrics extracted from 7n5c.cif.gz by aB_cif2table1 from BUSTER (Global Phasing Ltd.). See also the PDBx/mmCIF dictionary and a MXLIMS-compatible JSON representation.


Experimental information for 7N5C at RCSB, PDBe, PDBj

Experiment
Method
_exptl.method
X-RAY DIFFRACTION
Experimental protocol
_diffrn_radiation.pdbx_diffrn_protocol
SINGLE WAVELENGTH
Source type
_diffrn_source.source
SYNCHROTRON
Source details
_diffrn_source.type
AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site
_diffrn_source.pdbx_synchrotron_site
Australian Synchrotron
Beamline
_diffrn_source.pdbx_synchrotron_beamline
MX2
Temperature [K]
_diffrn.ambient_temp
100
Detector technology
_diffrn_detector.detector
PIXEL
Collection date
_diffrn_detector.pdbx_collection_date
2020-07-28
Detector
_diffrn_detector.type
DECTRIS EIGER X 16M
Wavelength(s) [Å]
_diffrn_source.pdbx_wavelength_list
0.987
Software
Data reduction
_software.classification
XDS
Data scaling
_software.classification
Aimless
Phasing
_software.classification
PHASER
Refinement
_software.classification
BUSTER (2.10.3)
General information
Spacegroup name
_symmetry.space_group_name_H-M
P 1 21 1
Unit cell parameters
_cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma}
57.086 74.113 114.295 90.00 99.34 90.00
Wavelength
_diffrn_radiation_wavelength.wavelength
0.98700 Å

Data quality metricsOverallInnerShellOuterShell
Low resolution limit [Å]
_reflns.d_resolution_low _reflns_shell.d_res_low
47.410 47.410 1.910
High resolution limit [Å]
_reflns.d_resolution_high _reflns_shell.d_res_high
1.870 9.350 1.870
Rmerge
_reflns.pdbx_Rmerge_I_obs _reflns_shell.Rmerge_I_obs
0.072 0.053 1.182
Rmeas
_reflns.pdbx_Rrim_I_all _reflns_shell.pdbx_Rrim_I_all
0.078 0.058 1.277
Rpim
_reflns.pdbx_Rpim_I_all _reflns_shell.pdbx_Rpim_I_all
0.029 0.023 0.480
Total number of observations
_reflns.pdbx_number_measured_all _reflns_shell.number_measured_all
546387 4432 32501
Total number unique
_reflns.number_obs _reflns_shell.number_unique_obs
77867 660 4626
<I/σ(I)>
_reflns.pdbx_netI_over_sigmaI _reflns_shell.pdbx_netI_over_sigmaI_obs
12.90 33.50 1.90
Completeness [%]
_reflns.percent_possible_obs _reflns_shell.percent_possible_all
100.0 99.3 100.0
Multiplicity
_reflns.pdbx_redundancy _reflns_shell.pdbx_redundancy
7.0 6.7 7.0
CC(1/2)
_reflns.pdbx_CC_half _reflns_shell.pdbx_CC_half
0.998 0.990 0.724

Refinement
PDB entry ID
_entry.id
7N5C
Deposition date
_pdbx_database_status.recvd_initial_deposition_date
2021-06-05
Resolution
_refine.ls_d_res_low _refine.ls_d_res_high
27.1 - 1.870 Å
Rwork/Rfree
_refine.ls_R_factor_R_work _refine.ls_R_factor_R_free
0.2040 / 0.2330
Structure solution method
_refine.pdbx_method_to_determine_struct
MOLECULAR REPLACEMENT
Starting model (for MR)
_refine.pdbx_starting_model
3PQY