| Experiment | |
|---|---|
Method _exptl.method | ELECTRON CRYSTALLOGRAPHY |
Experimental protocol _diffrn_radiation.pdbx_diffrn_protocol | SINGLE WAVELENGTH |
Source type _diffrn_source.source | ELECTRON MICROSCOPE |
Source details _diffrn_source.type | JEOL 4000 electron microscope |
Temperature [K] _diffrn.ambient_temp | 93 |
Detector technology _diffrn_detector.detector | CCD |
Collection date _diffrn_detector.pdbx_collection_date | 1994-01-01 |
Detector _diffrn_detector.type | GATAN |
Wavelength(s) [Å] _diffrn_source.pdbx_wavelength_list | 0.0194 |
| Software | |
Phasing _software.classification | CCP4 |
Model building _software.classification | CCP4 |
Refinement _software.classification | CNS (0.9) |
| General information | |
Spacegroup name _symmetry.space_group_name_H-M | P 1 21 1 |
Unit cell parameters _cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma} | 81.2 93.5 90.0 90.0 90.0 90.0 |
Wavelength _diffrn_radiation_wavelength.wavelength | 0.01940 Å |
| Data quality metrics | Overall |
|---|---|
Low resolution limit [Å] _reflns.d_resolution_low | 20.000 |
High resolution limit [Å] _reflns.d_resolution_high | 3.500 |
Rmerge _reflns.pdbx_Rmerge_I_obs | 0.250 |
| Rmeas | - |
| Rpim | - |
| Total number of observations | - |
Total number unique _reflns.number_obs | 12422 |
<I/σ(I)> _reflns.pdbx_netI_over_sigmaI | 5.40 |
Completeness [%] _reflns.percent_possible_obs | 73.0 |
Multiplicity _reflns.pdbx_redundancy | 6.0 |
| CC(1/2) | - |
| Refinement | |
|---|---|
PDB entry ID _entry.id | 1JFF |
Deposition date _pdbx_database_status.recvd_initial_deposition_date | 2001-06-20 |
Resolution _refine.ls_d_res_low _refine.ls_d_res_high | 20.0 - 3.500 Å |
Rwork/Rfree _refine.ls_R_factor_R_work _refine.ls_R_factor_R_free | 0.2315 / 0.2966 |
Structure solution method _refine.pdbx_method_to_determine_struct | MOLECULAR REPLACEMENT |
Starting model (for MR) _refine.pdbx_starting_model | PDB entry 1TUB |