Data quality metrics extracted from 8gm4.cif.gz by aB_cif2table1 from BUSTER (Global Phasing Ltd.). See also the PDBx/mmCIF dictionary and a MXLIMS-compatible JSON representation.


Experimental information for 8GM4 at RCSB, PDBe, PDBj

Experiment
Method
_exptl.method
X-RAY DIFFRACTION
Experimental protocol
_diffrn_radiation.pdbx_diffrn_protocol
SINGLE WAVELENGTH
Source type
_diffrn_source.source
SYNCHROTRON
Source details
_diffrn_source.type
NSLS-II BEAMLINE 17-ID-2
Synchrotron site
_diffrn_source.pdbx_synchrotron_site
NSLS-II
Beamline
_diffrn_source.pdbx_synchrotron_beamline
17-ID-2
Temperature [K]
_diffrn.ambient_temp
100
Detector technology
_diffrn_detector.detector
PIXEL
Collection date
_diffrn_detector.pdbx_collection_date
2022-05-28
Detector
_diffrn_detector.type
DECTRIS EIGER X 16M
Wavelength(s) [Å]
_diffrn_source.pdbx_wavelength_list
0.97934
Software
Data processing #1
_software.classification
autoPROC (1.0.5 20221121)
Data processing #2
_software.classification
TRUNCATE (7.1.018)
Data reduction
_software.classification
XDS (Jan 10, 2022 (BUILT 20220220))
Data scaling
_software.classification
Aimless (0.7.7)
Phasing
_software.classification
PHASER (1.20.1_4487)
Model building
_software.classification
Coot (0.9.6)
Refinement
_software.classification
PHENIX (1.20.1_4487)
General information
Spacegroup name
_symmetry.space_group_name_H-M
P 1 21 1
Unit cell parameters
_cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma}
80.544 60.684 88.704 90.0 111.1 90.0
Wavelength
_diffrn_radiation_wavelength.wavelength
0.97934 Å

Data quality metricsOverallInnerShellOuterShell
Low resolution limit [Å]
_reflns.d_resolution_low _reflns_shell.d_res_low
75.133 75.133 2.044
High resolution limit [Å]
_reflns.d_resolution_high _reflns_shell.d_res_high
2.009 5.452 2.009
Rmerge
_reflns.pdbx_Rmerge_I_obs _reflns_shell.Rmerge_I_obs
0.207 0.084 3.651
Rmeas
_reflns.pdbx_Rrim_I_all _reflns_shell.pdbx_Rrim_I_all
0.227 0.092 3.985
Rpim
_reflns.pdbx_Rpim_I_all _reflns_shell.pdbx_Rpim_I_all
0.092 0.038 1.583
Total number of observations
_reflns.pdbx_number_measured_all _reflns_shell.number_measured_all
320683 16913 16164
Total number unique
_reflns.number_obs _reflns_shell.number_unique_all
53518 2786 2638
<I/σ(I)>
_reflns.pdbx_netI_over_sigmaI _reflns_shell.meanI_over_sigI_obs
4.97 13.32 0.68
Completeness [%]
_reflns.percent_possible_obs _reflns_shell.percent_possible_all
100.0 99.9 100.0
Multiplicity
_reflns.pdbx_redundancy _reflns_shell.pdbx_redundancy
6.0 6.1 6.1
CC(1/2)
_reflns.pdbx_CC_half _reflns_shell.pdbx_CC_half
0.985 0.978 0.311
Anomalous completeness
_reflns.pdbx_percent_possible_anomalous _reflns_shell.pdbx_percent_possible_anomalous
99.3 99.6 99.4
Anomalous multiplicity
_reflns.pdbx_redundancy_anomalous _reflns_shell.pdbx_redundancy_anomalous
3.1 3.2 3.1
CC(ano)
_reflns.pdbx_CC_half_anomalous _reflns_shell.pdbx_CC_half_anomalous
-0.096 -0.089 -0.009
|DANO|/σ(DANO)
_reflns.pdbx_absDiff_over_sigma_anomalous _reflns_shell.pdbx_absDiff_over_sigma_anomalous
0.746 0.551 0.756

Refinement
PDB entry ID
_entry.id
8GM4
Deposition date
_pdbx_database_status.recvd_initial_deposition_date
2023-03-24
Resolution
_refine.ls_d_res_low _refine.ls_d_res_high
47.7 - 2.120 Å
Rwork/Rfree
_refine.ls_R_factor_R_work _refine.ls_R_factor_R_free
0.2071 / 0.2376
Structure solution method
_refine.pdbx_method_to_determine_struct
MOLECULAR REPLACEMENT
Starting model (for MR)
_refine.pdbx_starting_model
7SHQ