| Experiment | |
|---|---|
Method _exptl.method | X-RAY DIFFRACTION |
Experimental protocol _diffrn_radiation.pdbx_diffrn_protocol | MAD |
Source type _diffrn_source.source | SYNCHROTRON |
Source details _diffrn_source.type | APS BEAMLINE 23-ID-B |
Synchrotron site _diffrn_source.pdbx_synchrotron_site | APS |
Beamline _diffrn_source.pdbx_synchrotron_beamline | 23-ID-B |
Temperature [K] _diffrn.ambient_temp | 100 |
Detector technology _diffrn_detector.detector | CCD |
Collection date _diffrn_detector.pdbx_collection_date | 2008-03-19 |
Detector _diffrn_detector.type | MAR |
Wavelength(s) [Å] _diffrn_source.pdbx_wavelength_list | 0.9794, 0.9796 |
| Software | |
Data collection _software.classification | IDB |
Data scaling #1 _software.classification | SCALA (Phil R. Evans; pre@mrc-lmb.cam.ac.uk) |
Data scaling #2 _software.classification | XSCALE |
Phasing _software.classification | SHARP (Eric de La Fortelle; sharp-develop@globalphasing.com) |
Refinement _software.classification | CNS (1.2; Axel T. Brunger; axel.brunger@yale.edu) |
| General information | |
Spacegroup name _symmetry.space_group_name_H-M | P 41 21 2 |
Unit cell parameters _cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma} | 61.35 61.35 158.90 90.0 90.0 90.0 |
Wavelength _diffrn_radiation_wavelength.wavelength | 0.979400.97960 Å |
| Data quality metrics | Overall |
|---|---|
Low resolution limit [Å] _reflns.d_resolution_low | 19.804 |
High resolution limit [Å] _reflns.d_resolution_high | 2.200 |
Rmerge _reflns.pdbx_Rmerge_I_obs | 0.102 |
| Rmeas | - |
| Rpim | - |
| Total number of observations | - |
Total number unique _reflns.number_obs | 16129 |
| <I/σ(I)> | - |
Completeness [%] _reflns.percent_possible_obs | 97.1 |
Multiplicity _reflns.pdbx_redundancy | 7.7 |
| CC(1/2) | - |
| Refinement | |
|---|---|
PDB entry ID _entry.id | 3F0Z |
Deposition date _pdbx_database_status.recvd_initial_deposition_date | 2008-10-27 |
Resolution _refine.ls_d_res_low _refine.ls_d_res_high | 19.4 - 2.200 Å |
Rwork/Rfree _refine.ls_R_factor_R_work _refine.ls_R_factor_R_free | 0.1740 / 0.2170 |
Structure solution method _refine.pdbx_method_to_determine_struct | MAD |