Data quality metrics extracted from 8e8v.cif.gz by aB_cif2table1 from BUSTER (Global Phasing Ltd.). See also the PDBx/mmCIF dictionary and a MXLIMS-compatible JSON representation.


Experimental information for 8E8V at RCSB, PDBe, PDBj

Experiment
Method
_exptl.method
X-RAY DIFFRACTION
Experimental protocol
_diffrn_radiation.pdbx_diffrn_protocol
SINGLE WAVELENGTH
Source type
_diffrn_source.source
SYNCHROTRON
Source details
_diffrn_source.type
NSLS-II BEAMLINE 17-ID-1
Synchrotron site
_diffrn_source.pdbx_synchrotron_site
NSLS-II
Beamline
_diffrn_source.pdbx_synchrotron_beamline
17-ID-1
Temperature [K]
_diffrn.ambient_temp
100
Detector technology
_diffrn_detector.detector
PIXEL
Collection date
_diffrn_detector.pdbx_collection_date
2022-06-16
Detector
_diffrn_detector.type
DECTRIS EIGER X 9M
Wavelength(s) [Å]
_diffrn_source.pdbx_wavelength_list
0.92010
Software
Data reduction
_software.classification
autoPROC
Data scaling
_software.classification
Aimless (0.7.4)
Phasing
_software.classification
PHASER
Refinement
_software.classification
PHENIX (1.14_3260)
General information
Spacegroup name
_symmetry.space_group_name_H-M
P 1 21 1
Unit cell parameters
_cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma}
77.099 155.963 78.352 90.0 100.2 90.0
Wavelength
_diffrn_radiation_wavelength.wavelength
0.92010 Å

Data quality metricsOverallInnerShellOuterShell
Low resolution limit [Å]
_reflns.d_resolution_low _reflns_shell.d_res_low
47.490 47.490 2.510
High resolution limit [Å]
_reflns.d_resolution_high _reflns_shell.d_res_high
2.450 11.490 2.450
Rmerge
_reflns.pdbx_Rmerge_I_obs _reflns_shell.Rmerge_I_obs
0.148 0.069 2.378
Rmeas
_reflns.pdbx_Rrim_I_all _reflns_shell.pdbx_Rrim_I_all
0.164 0.077 2.644
Rpim
_reflns.pdbx_Rpim_I_all _reflns_shell.pdbx_Rpim_I_all
0.071 0.033 1.144
Total number of observations
_reflns.pdbx_number_measured_all _reflns_shell.number_measured_all
350595 3412 23252
Total number unique
_reflns.number_obs _reflns_shell.number_unique_obs
66842 670 4452
<I/σ(I)>
_reflns.pdbx_netI_over_sigmaI _reflns_shell.pdbx_netI_over_sigmaI_obs
6.50 19.10 0.80
Completeness [%]
_reflns.percent_possible_obs _reflns_shell.percent_possible_all
99.9 98.7 99.2
Multiplicity
_reflns.pdbx_redundancy _reflns_shell.pdbx_redundancy
5.2 5.1 5.2
CC(1/2)
_reflns.pdbx_CC_half _reflns_shell.pdbx_CC_half
0.994 0.992 0.428

Refinement
PDB entry ID
_entry.id
8E8V
Deposition date
_pdbx_database_status.recvd_initial_deposition_date
2022-08-25
Resolution
_refine.ls_d_res_low _refine.ls_d_res_high
47.5 - 2.450 Å
Rwork/Rfree
_refine.ls_R_factor_R_work _refine.ls_R_factor_R_free
0.2340 / 0.2578
Structure solution method
_refine.pdbx_method_to_determine_struct
MOLECULAR REPLACEMENT
Starting model (for MR)
_refine.pdbx_starting_model
8E8T