Data quality metrics extracted from 3bem.cif.gz by aB_cif2table1 from BUSTER (Global Phasing Ltd.). See also the PDBx/mmCIF dictionary and a MXLIMS-compatible JSON representation.


Experimental information for 3BEM at RCSB, PDBe, PDBj

Experiment
Method
_exptl.method
X-RAY DIFFRACTION
Experimental protocol
_diffrn_radiation.pdbx_diffrn_protocol
MAD
Source type
_diffrn_source.source
SYNCHROTRON
Source details
_diffrn_source.type
APS BEAMLINE 23-ID-D
Synchrotron site
_diffrn_source.pdbx_synchrotron_site
APS
Beamline
_diffrn_source.pdbx_synchrotron_beamline
23-ID-D
Temperature [K]
_diffrn.ambient_temp
100
Detector technology
_diffrn_detector.detector
CCD
Collection date
_diffrn_detector.pdbx_collection_date
2007-10-26
Detector
_diffrn_detector.type
MARMOSAIC 300 mm CCD
Wavelength(s) [Å]
_diffrn_source.pdbx_wavelength_list
0.91840, 0.97939, 0.97953
Software
Data collection
_software.classification
MAR345 (CCD)
Data reduction
_software.classification
XDS
Data scaling
_software.classification
XSCALE (Wolfgang Kabsch)
Phasing
_software.classification
SOLVE (Tom Terwilliger; terwilliger@LANL.gov)
Model building
_software.classification
MolProbity (3beta29; D.C. & J.S. Richardson lab; molprobity@kinemage.biochem.duke.edu)
Refinement #1
_software.classification
REFMAC (5.2.0019; Murshudov, G.N.; ccp4@dl.ac.uk)
Refinement #2
_software.classification
PHENIX (P.D. Adams; PDAdams@lbl.gov)
General information
Spacegroup name
_symmetry.space_group_name_H-M
P 1 21 1
Unit cell parameters
_cell.length_{a,b,c} _cell.angle_{alpha,beta,gamma}
51.62 69.20 61.85 90.00 100.54 90.00
Wavelength
_diffrn_radiation_wavelength.wavelength
0.918400.979390.97953 Å

Data quality metricsOverallInnerShellOuterShell
Low resolution limit [Å]
_reflns.d_resolution_low _reflns_shell.d_res_low
28.583 28.583 1.710
High resolution limit [Å]
_reflns.d_resolution_high _reflns_shell.d_res_high
1.650 3.550 1.650
Rmerge
_reflns.pdbx_Rmerge_I_obs _reflns_shell.Rmerge_I_obs
0.051 0.036 0.291
  Rmeas - - -
  Rpim - - -
Total number of observations
_reflns_shell.number_measured_obs
- 17785 16811
Total number unique
_reflns.number_obs _reflns_shell.number_unique_obs
51334 9996 9336
<I/σ(I)>
_reflns.pdbx_netI_over_sigmaI _reflns_shell.meanI_over_sigI_obs
8.98 17.90 2.90
Completeness [%]
_reflns.percent_possible_obs _reflns_shell.percent_possible_all
97.0 97.2 89.9
  Multiplicity - - -
  CC(1/2) - - -

Refinement
PDB entry ID
_entry.id
3BEM
Deposition date
_pdbx_database_status.recvd_initial_deposition_date
2007-11-19
Resolution
_refine.ls_d_res_low _refine.ls_d_res_high
28.6 - 1.650 Å
Rwork/Rfree
_refine.ls_R_factor_R_work _refine.ls_R_factor_R_free
0.1510 / 0.1750
Structure solution method
_refine.pdbx_method_to_determine_struct
MAD